THE ROLE OF PHYTOHORMONES TO ENHANCE THE EXPRESSION OF LIPID TRANSFER PROTEIN3 (LTP3) IN IMPROVING STAPLE LENGTH OF Gossypium hirsutum L.
Here is a abstract that i had submitted at the Plant & Animal Genomes XI Conference January 11-15, 2003 Town & Country Convention Center San Diego, CA. I do not know how relevant is the issue to this day. If any one is interested or if any one is working in these lines, do let me know also pour in your suggestions and comments if any.
Fiber quality in cotton is one very vital trait demanding international focus in both scientific and commercial arena. Phytohormones play a major role in all cellular processes in plant development. We have focused our study on the influence of phytohormones in improving the staple length of cotton fiber. There are several pathways, which influence staple length in cotton, among which we have concentrated on the expression pattern of Lipid Transfer Protein3. A cotton fiber protein gene Ltp3, Lipid transfer protein3 (coding for a LTP3 protein), is specifically expressed in fibers at high levels, during the elongation stage. Its levels reach a maximum during the late elongation stage of fiber development and dramatically decrease in the end of late elongation stage (secondary wall synthesis stage). GH3 (Growth Hormone) gene, which is a complementary-DNA plasmid of the Ltp3 gene, is Phytohormone responsive. We have developed the 3D model of LTP3 using MODELLER and trail version of Molecular Operating Environment (MOE). We propose to study In silico, the protein-protein interaction and expression profiles of LTP3 and other proteins involved in staple length improvement. Further study is underway to establish the mechanistic role of phytohormones in effecting LTP3 expression thereby influencing the staple length in cotton.
Fiber quality in cotton is one very vital trait demanding international focus in both scientific and commercial arena. Phytohormones play a major role in all cellular processes in plant development. We have focused our study on the influence of phytohormones in improving the staple length of cotton fiber. There are several pathways, which influence staple length in cotton, among which we have concentrated on the expression pattern of Lipid Transfer Protein3. A cotton fiber protein gene Ltp3, Lipid transfer protein3 (coding for a LTP3 protein), is specifically expressed in fibers at high levels, during the elongation stage. Its levels reach a maximum during the late elongation stage of fiber development and dramatically decrease in the end of late elongation stage (secondary wall synthesis stage). GH3 (Growth Hormone) gene, which is a complementary-DNA plasmid of the Ltp3 gene, is Phytohormone responsive. We have developed the 3D model of LTP3 using MODELLER and trail version of Molecular Operating Environment (MOE). We propose to study In silico, the protein-protein interaction and expression profiles of LTP3 and other proteins involved in staple length improvement. Further study is underway to establish the mechanistic role of phytohormones in effecting LTP3 expression thereby influencing the staple length in cotton.
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