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Monday, March 26, 2012

Disulphide Redox Metabolic Database of genus Listeria

Listeria TDRM Database Please keep your comments and suggestions flowing in

The genus Listeria consists of a group of gram-positive bacteria of low G+C content closely related to Bacillus, Clostridium, Enterococcus, Streptococcus, and StaphylococcusListeria spp. are facultative anaerobic rods of 0.4 by 1 to 1.5 µm that do not form spores, have no capsule and are motile at 10 to 25°C. Listeria spp. is isolated from a diversity of environmental sources, including soil, water, effluents, a large variety of foods, and the feces of humans and animals. The genus Listeria currently includes eight species: L. monocytogenesL. ivanoviiL. seeligeri,L.rocourtiaceL. innocuaL. welshimeriL. marthii and L. grayi. Two of these species L. monocytogenes and L. ivanovii are potentially pathogenic. The infectious disease caused by these bacteria is known as listeriosis. L. monocytogenes causes serious localized and generalized infections in humans and a variety of other vertebrates, including domesticated and wild birds and mammals. Human cases of L. ivanovii infection are rare the vast majority of reported isolations of this species being from abortions, stillbirths and neonatal septicemias in sheep and cattle. A third species L. seeligeri is considered nonpathogenic. Recently L. grayi DSM 20601 has been reportedinfectious to immmunocompromised patients.

Synthesis of glutathione a low-molecular-weight peptide thiol was thought to occur solely through the consecutive action of two physically separate enzymes, gamma-glutamylcysteine ligase and glutathione synthetase. But it has been identified that L. monocytogenes contains a novel multidomain protein (termed GshF) that carries out complete synthesis of glutathione. It is a 776 amino acid fusion protein and fusion occurs in 338 to 580 amino acids.It is coded by the gene Lmo 2770 in L. monocytogenes.

Thiol:disulfide redox metabolism (TDRM) is a central metabolic network in all living cells. In L.monocytogenes, we have identified 26 genes that belong thiol disulphide network. L. monocytogenes-Thiol: Disulphide Redox Metabolism database provides information on Thiol disulphide redox gene and gene products. This database also provides information about the Gene and Domain Annotation, Clustering, Structure Details. TDRM analysis gives detailed analysis of the similar cluster of these TDRM genes across bacterial species consisting 82 bacterial strains. With the help genome browser, user can view the TDRM genes on the genomic scale and analyze the same.

  1. Understanding TDRM genes on the genomic scale with reference to other GshF containing bacteria
  2. Finding the genes which have probable role in thiolomics in L. monocytogenes EGD-e
  3. Genetic context of the relevant genes in Listeria with other bacteria and their annotations

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Life Science and Informatics

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These are some interesting things that could be discussed in this blog page...

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